Chromatography testing is a method used
by a wide variety of industries for many different purposes. Chromatography
essentially represents an array of laboratory techniques for the separation of
mixtures. During the test, the chemist will pass a mixture dissolved in
a mobile phase through a stationary phase, to separate whatever is being analyzed
from the other molecules in the mixture. This procedure allows the molecule
being measured to be isolated.
The mobile phase of the testing is a liquid or gas. The stationary phase is a solid or liquid contained in a column, on paper or in a thin layer on a glass support. In this method of testing, the elements of the mixture are either absorbed or hindered by the stationary phase to varying degrees and therefore become separated.
There are two main types of chromatography testing: Preparative is typically conducted on a larger scale for the purification and collection of one or more of a blends components.
Analytical is generally conducted on smaller quantities to quantify and identify the component parts of the blend. Most people are familiar with using analytical chromatography testing for determining the alcohol content in blood and urine samples.
A market leader in chromatography testing
is Galbraith Laboratories, offering over 60 years relevant experience in a variety
of industries. This world-class organization offers four main chromatography
testing methods: High Performance Liquid Chromatography (HPLC) Frequently used in analytical chemistry, this method separates the components of a mixture using a variety of chemical interactions between the compounds with the analyzed substance and the chromatography column.
Gas Chromatography (GC) A chemical analysis method used to separate volatile and semi-volatile compounds in a sample. Often used when only a small quantity of the sample is available and for substances that do not decompose at high temperatures. Ion Chromatography (IC) This method uses ion exchange resins to separate atomic or molecular ions based on their interaction with the resin.
Thin Layer Chromatography (TLC) This technique separates and analyzes mixtures of substances using a thin layer of stationary phase attached to a glass plate and for the mobile phase utilizing the passage of liquid up the plate.